Hyper-secretion mechanism exploration of a heterologous creatinase in Bacillus subtilis

Zhengyu Tao,Gang Fu,Sijia Wang,Zhaoxia Jin,Jianping Wen,Dawei Zhang

Biochemical Engineering Journal（2020）

引用 3|浏览17

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摘要

•Creatinase activity in B. subtilis was up to 141.9 U/mL by high-density fermentation.•Calcein-AM/PI assays confirmed the cell membrane was damaged.•TEM and SEM results found the presence of leakage site on the bacterial cell surface.

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关键词

Creatinase,Bacillus subtilis,Heterologous expression,Leakage-based expression,High-density fermentation,Promoter optimization

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