Soy extract as protein replacement to feed Melipona flavolineata Friese (Hymenoptera, Apidae, Meliponini)

The study of the feeding of bees is important because periods of flowering shortages reduce the availability of food, jeopardising their survival and limiting the production of their colonies. Previous studies on artificial protein feeding have not reported the chemical composition of the artificial feed, its effect on the colony nor the characterisation of the food. Thus, the present research aimed to study an artificial proteic feed for Melipona flavolineata. We investigated its chemical composition and the effect of natural fermentation on its quality. We also investigated its effects on colony and individual health. The artificial food presented a chemical composition different from natural pollen. It was richer in carbohydrates and lipids than natural pollen, but it was poorer in protein than natural pollen. Nevertheless, the difference between the artificial food and natural pollen did not affect the survival or size of immature individuals, with only adult longevity being affected, albeit not drastically. Decreased amounts of sugar and the addition of ingredients with higher protein content are recommended for future formulations. A diet based on soy extract showed satisfactory results as a food substitute for pollen. Based on these results, diet ingredients can be adjusted to obtain a better-quality food. Artificial diets can be used during scarcity periods or in areas with low availability of natural food sources. However, it is important to emphasize that artificial diets will never fully replace natural food sources. Beekeepers should also improve the environment with plants that provide pollen continuously.