RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance.

H A Walsh, H Vanderschuren, S Taylor,M E C Rey

Biotechnology Reports（2019）

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摘要

•A hairpin-RNA construct targeting South African cassava mosaic virus AC1/AC4 overlapping genome region confers tolerance in cassava.•Viral load and symptom severity were significantly lower in transgenic lines transformed with AC1/AC4 construct compared to wildtype cv.60444.•SACMV transgenic lines infected with SACMV displayed a recovery phenotype similar to cassava mosaic disease-tolerant cassava landrace TME3.

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关键词

SACMV,siRNA,AC1,AC4,CMD,hp-RNA,VSR

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