Resveratrol addition to CHO cell culture media: The effect on cell growth, monoclonal antibody synthesis and its chemical modification.

The effect of the addition of resveratrol to cell culture media during the production of monoclonal antibodies was investigated. Treatments of Chinese hamster ovary (CHO) cells expressing immunoglobulin G (IgG) with 25 and 50 mu M resveratrol showed that resveratrol was capable of slowing cell growth while almost doubling cell-specific productivity to 4.7 +/- 0.6 pg IgG/cell center dot day, resulting in up to a 1.37-fold increase of the final IgG titer. A resveratrol concentration of 50 mu M slowed the progression through the cell cycle temporarily by trapping cells in the S-phase. Cation exchange chromatography showed no significant difference in the composition of acidic or basic IgG species and size exclusion chromatography indicated no change in fragmentation or aggregation of the recombinant IgG in the treatment groups. Resveratrol could be used as a chemical additive to CHO media where it would enhance IgG productivity and provide a degree of protection against hydroxyl and superoxide free radicals, expanding the range of options for process improvement available to monoclonal antibody manufacturers.