The lytic bacteriophage vB_EfaH_EF1TV, a new member of the Herelleviridae family, disrupts biofilm produced by Enterococcus faecalis clinical strains.

•EF1TV is a newly discovered lytic bacteriophage specific for Enterococcus faecalis.•Phage EF1TV is the fourth characterized member of the Kochikohdavirus genus within the Herelleviridae family.•EF1TV disrupts biofilm produced by strains of Enterococcus faecalis, even if they are not able to produce productive infection.•Integrity of the enterococcal polysaccharide antigen gene cluster is necessary for the infectivity of EF1TV.