Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.

•Native chitin (Chs5) and glucan synthase (Gsc1) visualised in the pathogen Zymoseptoria tritici.•Chs5 and Gsc1 are transported along microtubules.•Chs5 and Gsc1 do localise to the apical plasma membrane, but not the Spitzenkörper.•Light and electron microscopy how co-travel of Chs5 and Gsc1 in the same secretory vesicle.•Enzyme delivery in Z. tritici is different from Neurospora crassa, but similar to Ustilago maydis.