Sequence-Based Saudi Population Data For The Se33 Locus

A set of 87 reference samples collected from the population of Saudi Arabia were sequenced using the ForenSeq (TM) DNA Signature Prep Kit on a MiSeq FGx (TM). The FASTQ files contain the sequences of the SE33 STR, but are not reported by the ForenSeq (TM) Universal Analysis Software (UAS). The STRait Razor software was used to recover and to report SE33 sequence-based data for the Saudi population. Ninety-six sequence-based alleles were recovered, most of which had previously reported motif patterns. Two unreported motif patterns found in three alleles and seven novel allele sequences were reported. We also reported a single discordance between the sequence-based data and the CE data that was due to the presence of a common rrn deletion. SE33 had 130% more sequence-based alleles; the highest number of observed sequence variants were in alleles 27.2 and 30.2, which each had 7 sequence variants. The statistical parameters emphasize the usefulness of using the sequence-based data.