Cytotoxic effects of Tetraselmis suecica chloroform extracts with silver nanoparticle co-application on MCF-7, 4 T1, and Vero cell lines
Journal of Applied Phycology(2019)
摘要
In the present study we evaluated the effects of silver nanoparticles (AgNPs) and Tetraselmis suecica chloroform (CHL) crude extracts as single and co-applications against MCF-7 and 4 T1 breast cancer cells and normal Vero cell-lines. The AgNPs single application exhibited the highest cytotoxicity in a dose-dependent manner with IC 50 of 5.3, 17.78, and 25.11 μg mL −1 against MCF-7, 4 T1 and Vero cell lines, respectively, after 72 h treatments. The AgNPs- T. suecica -CHL co-application at 2:1 ratio achieved the IC 50 of 6.60 and 53.7 μg mL −1 on MCF-7 and 4 T1 cells, respectively, while the T. suecica -CHL single application only showed the IC 50 of 46.77 and 83.17 μg mL −1 , respectively. However, both the T. suecica -CHL and AgNPs- T. suecica -CHL showed no cytotoxic activity against the Vero cells. The AgNPs- T. suecica -CHL exhibited the highest late apoptotic events on MCF-7 (38.8%), followed by Tamoxifen (TMX), AgNPs and T. suecica -CHL. The cell cycle analysis of AgNPs- T. suecica -CHL–treated cells showed a significant increase in the accumulation of events at sub-G1 phase with increased ADP/ATP ratio and Caspase 3/7, suggesting the induction of apoptosis. The results brought new insights into the formulation of microalgal crude extracts (MCEs) and AgNPs co-applications in exerting strong cytotoxic effects on MCF-7 and 4 T1 cancer cells, but without having any cytotoxicity on the normal Vero cell-lines.
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关键词
Microalgae, Natural product, Chlorophyta,
Tetraselmis suecica
, Silver nanoparticles, Co-application, Anti-cancer agent
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