Optimisation of bioimpedance measurements of neuronal activity with an ex vivo preparation of Cancer pagurus peripheral nerves

•Physiological parameters for the crab walking leg nerve model of an unmyelinated fibre were optimised resulting in improved nerve health.•Developed protocol to combine improved bioimpedance measurement methods with previously reported unmyelinated fibre recording methods.•New, optimised method produced recorded data with single trace SNR ratios of ≥3:1, which resulted in a 10-trace average of an SNR of ≥9:1.•Demonstrated two methods in which to ascertain if the measured bioimpedance change is a genuine CAP dependent signal.