‘Off–on’ switchable fluorescent probe for prompt and cost-efficient detection of bacteria
NEW JOURNAL OF CHEMISTRY(2019)
摘要
The rapid and straightforward detection of bacteria in food and human samples is becoming important, particularly in view of the development of point-of-care devices and lab-on-a-chip tools for prevention and treatment of bacterial infections. In the present work, we present the design of a cost-efficient, switchable, fluoresce-based probe for the rapid detection of glucosidase-producing bacteria. The probe was prepared by functionalising streptavidin-coated magnetic particles with a switchable substrate capable of indicating the presence of the metabolic enzyme glucosidase (Glu-C-MPs). Under normal conditions the probe is 'off' but is switched 'on' in the presence of glucosidase-producing bacteria, leading to a measurable increase in fluorescence, indicating the presence of bacteria in the sample. The selectivity of the probe was firstly evaluated using enzymatic experiments, showing that it can detect both alpha- and beta-glucosidase. Subsequently, we tested the efficacy of the probe in detecting bacteria using microorganisms that tend to infect the urinary tract, inducing over-active bladder (OAB) in women. The probe successfully detected the presence of E. coli, K. pneumoniae and P. aeruginosa at low concentrations (75 CFU), within 60 minutes and with a good signal-to-noise ratio. 3 hours were required to observe an increase in fluorescence in the presence of Enterococcus and S. aureus, indicating that these microorganisms produce lower amounts of the detected enzyme. Glu-C-MPs are a promising tool for the prompt, easy and cost-efficient detection of microorganisms, demonstrating a high selectivity and sensitivity for glucosidase-producing bacteria. Thanks to the simple readout and the low-cost instrumentation required for the analysis, the proposed approach could pave the way for the development of affordable point-of-care devices.
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关键词
switchable fluorescent probe,bacteria,detection,cost-efficient
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