F-18-Fac Pet Visualizes Brain-Infiltrating Leukocytes In A Mouse Model Of Multiple Sclerosis

Brain-infiltrating leukocytes contribute to multiple sclerosis (MS) and autoimmune encephalomyelitis and likely play a role in traumatic brain injury, seizure, and stroke. Brain-infiltrating leukocytes are also primary targets for MS disease-modifying therapies. However, no method exists for noninvasively visualizing these cells in a living organism. 1-(2'deoxy-2'-18F-fluoroarabinofuranosyl) cytosine (F-18-FAC) is a PET radiotracer that measures deoxyribonucleoside salvage and accumulates preferentially in immune cells. We hypothesized that F-18-FAC PET could noninvasively image brain-infiltrating leukocytes. Methods: Healthy mice were imaged with F-18-FAC PET to quantify if this radiotracer crosses the blood brain barrier (BBB). Experimental autoimmune encephalomyelitis (EAE) is a mouse disease model with brain-infiltrating leukocytes. To determine whether F-18-FAC accumulates in brain-infiltrating leukocytes, EAE mice were analyzed with F-18-FAC PET, digital autoradiography, and immunohistochemistry, and deoxyribonucleoside salvage activity in brain-infiltrating leukocytes was analyzed ex vivo. Fingolimod-treated EAE mice were imaged with F-18-FAC PET to assess if this approach can monitor the effect of an immunomodulatory drug on brain-infiltrating leukocytes. PET scans of individuals injected with 2-chloro-2'-deoxy-2'-F-18-fluoro-9-13-q-arabinofuranosyl-adenine (F-18-CFA), a PET radiotracer that measures deoxyribonucleoside salvage in humans, were analyzed to evaluate whether F-18-CFA crosses the human BBB. Results: F-18-FAC accumulates in the healthy mouse brain at levels similar to F-18-FAC in the blood (2.54 +/- 0.2 and 3.04 +/- 0.3 percentage injected dose per gram, respectively) indicating that F-18-FAC crosses the BBB. EAE mice accumulate F-18-FAC in the brain at 180% of the levels of control mice. Brain F-18-FAC accumulation localizes to periventricular regions with significant leukocyte infiltration, and deoxyribonucleoside salvage activity is present at similar levels in brain infiltrating T and innate immune cells. These data suggest that F-18-FAC accumulates in brain-infiltrating leukocytes in this model. Fingolimodtreated EAE mice accumulate F-18-FAC in the brain at 37% lower levels than control-treated EAE mice, demonstrating that F-18-FAC PET can monitor therapeutic interventions in this mouse model. F-18-CFA accumulates in the human brain at 15% of blood levels (0.08 +/- 0.01 and 0.54 0.07 SUV, respectively), indicating that F-18-CFA does not cross the BBB in humans. Conclusion: F-18-FAC PET can visualize brain-infiltrating leukocytes in a mouse MS model and can monitor the response of these cells to an immunomodulatory drug. Translating this strategy into humans will require exploring additional radiotracers.