Reliability of a Single-region Sample to evaluate Immune Tumor Microenvironment in Hepatocellular Carcinoma.

•Most HCC tumors displayed similar tumor immune microenvironments among different regions within each tumor.•A single-region sample might be reliable to evaluate the tumor immune microenvironment of the entire HCC tumor.•Most HCC tumors displayed uniform expression of PD-L1 in different regions within each tumor.•Intratumor tertiary lymphoid structures are rare in HCC, and are prone to spatial heterogeneity.