Chitiniphilus Eburneus Sp. Nov., A Novel Chitinolytic Bacterium Isolated From Sludge

A novel Gram-stain-negative, curved rod-shaped, motile and non-endospore-forming strain, designated HX-2-15(T), was isolated from activated sludge of agricultural chemical plant in Nanjing, Jiangsu province, PR China (32 degrees 03' N, 118 degrees 46' E). Growth was observed at 15-37 degrees C (optimum between 25 and 30 degrees C), at pH 6.0-8.0 (optimum at pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum at 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed closest affiliation to Chitiniphilus shinanonensis SAY3(T), with a sequence similarity of 99.0 %. The predominant cellular fatty acids were C-16:0, C-17:0 cyclo and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). The major quinone was ubiquinone Q-8. The polar lipid profile was composed of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified lipid and one unidentified aminophosphoglycolipid. The genomic DNA G+C content of the strain was 63.6 mol%. The ANI and dDDH values obtained between the genomes of HX-2-15(T) and C. shinanonensis SAY3(T) were 85.3 and 29.3 % respectively. On the basis of data from phenotypic, chemotaxonomic and genotypic analysis, strain HX-2-15(T) represents a novel species of the genus Chitiniphilus, for which the name Chitiniphilus eburneus sp. nov. is proposed. The type strain is HX-2-15(T) (=KCTC 72286(T)=CCTCC AB 2019178(T)).