N-halamine polyelectrolytes used for preparation of antibacterial polypropylene nonwoven fabrics and study on their basal cytotoxicity and mutagenicity

Polymeric N-halamine precursors, cationic poly((3-acrylamidopropyl) trimethylammonium chloride) (CHP) and anionic poly(2-acrylamido-2-methylpropane sulfonic acid sodium salt) (AHP), were synthesized and coated onto polypropylene nonwoven fabrics via eco-friendly dipping and spraying methods. The successful coatings were characterized by EDS analysis. After exposure to diluted sodium hypochlorite solution, the coatings became biocidal. Antibacterial tests showed that PP-Dip-Cl samples could inactivate more than 5 logs of Staphylococcus aureus and Escherichia coli O157:H7 within 60 and 30 min, respectively. Although PP-Spray-Cl samples had a relatively low active chlorine content, all inoculated bacteria were inactivated within 60 min for both S. aureus and E. coli O157:H7. All the coatings showed proper storage stability under dark and lab-light conditions. The coated samples showed good air permeability. Basal cytotoxicity test showed that the estimated acute oral LD50 value was 209 +/- 14 mg/kg b. w. and 353 +/- 14 mg/kg b. w. for CHP-Cl and AHP-Cl, respectively and no indication of mutagenicity was observed with and without external metabolizing enzyme system (rat liver S9 mix) for both the two N-halamine compounds. The eco-friendly coatings have great potential for applications in hospitals. [GRAPHICS] .