Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach.

Laura Hebberecht,Leen Vancoillie,Marlies Schauvliege,Delfien Staelens,Els Demecheleer, Jarryt Hardy,Virginie Mortier,Chris Verhofstede

Journal of Virological Methods（2019）

引用 4|浏览8

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摘要

•Highly sensitive protocol for sequencing of near full-length HIV-1 RNA.•Single variant full genome amplification using a two-fragment strategy.•Limiting dilution of HIV-1 RNA as a way to reduce in vitro recombination.

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关键词

Near full-length HIV-1 sequencing,Single genome sequencing,HIV-1 RNA limiting dilution,Recombination

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