Il-1 Beta Enhances Cell Adhesion Through Laminin 5 And Integrin Beta 4 Ingingival Epithelial Cells

The junctional epithelium and dental enamel adhere because of hemidesmosomes containing laminin 5 and alpha 6 beta 4 integrin, which are important adhesion molecules in the internal basal lamina. Interleukin (IL)-1 is important in the pathogenesis of periodontal disease. IL-1 beta induces bone resorption by activating osteoclasts; however, its effects on adhesion of epithelial cells remain to be clarified. Laminin beta 3, beta 4 integrin, and focal adhesion kinase mRNA levels were higher after 1 h and 3 h of stimulation with IL-1 beta (1 ng/mL), and IL-1 beta type I alpha 1, and type IV alpha 1 collagen mRNA levels were higher after 1 h and lower after 3 h of stimulation with After IL-beta 1 stimulation, colocalization of laminin 5 and beta 4 integrin was increased after 1 h, colocalization of beta 4 integrin and plectin was increased after 1 h and decreased after 3 h, and colocalization of beta 4 integrin and type IV collagen was decreased after 3 h. Wound healing assays showed that IL-1 beta treatment (3 h) delayed wound healing. These results suggest that IL-1 beta enhances cell adhesion by altering localization of epithelial adhesion molecules.