Transcriptome analysis of Globodera pallida from the susceptible host Solanum tuberosum or the resistant plant Solanum sisymbriifolium

A transcriptome analysis of G . pallida juveniles collected from S . tuberosum or S . sisymbriifolium 24 h post infestation was performed to provide insights into the parasitic process of this nematode. A total of 41 G . pallida genes were found to be significantly differentially expressed when parasitizing the two plant species. Among this set, 12 were overexpressed when G . pallida was parasitizing S . tuberosum and 29 were overexpressed when parasitizing S . sisymbriifolium . Out of the 12 genes, three code for secretory proteins; one is homologous to effector gene Rbp-4, the second is an uncharacterized protein with a signal peptide sequence, and the third is an ortholog of a Globodera rostochiensis effector belonging to the 1106 effector family. Other overexpressed genes from G . pallida when parasitizing S . tuberosum were either unknown, associated with a stress or defense response, or associated with sex differentiation. Effector genes namely Eng-1, Cathepsin S-like cysteine protease, cellulase, and two unknown genes with secretory characteristics were over expressed when G . pallida was parasitizing S . sisymbriifolium relative to expression from S . tuberosum . Our findings provide insight into gene regulation of G . pallida while infecting either the trap crop S . sisymbriifolium or the susceptible host, S . tuberosum .