Extracellular RNA in a single droplet of human serum reflects physiologic and disease states

Zixu Zhou
Zixu Zhou
Qiuyang Wu
Qiuyang Wu
Zhangming Yan
Zhangming Yan
Haizi Zheng
Haizi Zheng
Chien-Ju Chen
Chien-Ju Chen
Yuan Liu
Yuan Liu
Zhijie Qi
Zhijie Qi
Riccardo Calandrelli
Riccardo Calandrelli
Zhen Chen
Zhen Chen
H Irene Su
H Irene Su

Proceedings of the National Academy of Sciences of the United States of America, pp. 19200-19208, 2019.

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Keywords:
agebiomarkerbreast cancercancer recurrenceextracellular RNA

Abstract:

Extracellular RNAs (exRNAs) are present in human serum. It remains unclear to what extent these circulating exRNAs may reflect human physiologic and disease states. Here, we developed SILVER-seq (Small Input Liquid Volume Extracellular RNA Sequencing) to efficiently sequence both integral and fragmented exRNAs from a small droplet (5 μL t...More

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