Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules.

Deepak B Thimiri Govinda Raj,Niamat Ali Khan,Srisaran Venkatachalam,Sivakumar Arumugam, Selvamani Palaniswamy

Methods in molecular biology (Clifton, N.J.)（2019）

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摘要

Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.

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关键词

AuNPs,Bio-conjugation,Phospholipids,SPMNPs

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