Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods.

•For the first time 21 B-vitamins were quantified from miniscule volume of milk.•Three different fresh milk samples from cow, goat, buffalo were compared.•Total choline is the highest and vitamin-B8 is the lowest in all milk samples.•Inverse correlation between vitamin B2 and B5 was observed in milk samples.•Total B-vitamin and B12 contents are higher in cow milk compared to other two.