A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

Background In most microbial cultivations d -glucose is the main carbon and energy source. However, quantification of d -glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose biosensor, which can be applied in microbioreactor-based cultivations. This sensor consists of a glucose binding protein sandwiched between two fluorescent proteins, constituting a FRET pair. Upon d -glucose binding the sensor undergoes a conformational change which is translated into a FRET-ratio change. Results The selected sensor shows an apparent K d below 1.5 mM d -glucose and a very high sensitivity of up to 70% FRET-ratio change between the unbound and the glucose-saturated state. The soluble sensor was successfully applied online to monitor the glucose concentration in an Escherichia coli culture. Additionally, this sensor was utilized in an at-line process for a Corynebacterium glutamicum culture as an example for a process with cell-specific background (e.g. autofluorescence) and medium-induced quenching. Immobilization of the sensor via HaloTag ® enabled purification and covalent immobilization in one step and increased the stability during application, significantly. Conclusion A FRET-based glucose sensor was used to quantify d -glucose consumption in microtiter plate based cultivations. To the best of our knowledge, this is the first method reported for online quantification of d -glucose in microtiter plate based cultivations. In comparison to d -glucose analysis via an enzymatic assay and HPLC, the sensor performed equally well, but enabled much faster measurements, which allowed to speed up microbial strain development significantly.