Kribbella Jiaozuonensis Sp. Nov., A Novel Actinomycete Isolated From Soil

A novel actinobacterium, designated strain NEAU-THZ27(T), was isolated from soil collected from the Cornet peak in Jiaozuo, Henan Province, PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genusKribbella. The results of 16S rRNA gene sequence analysis showed that strain NEAU-THZ27(T) belongs to the genus Kribbella and was most closely related to Kribbella podocarpi YPL1(T) (98.96 %), Kribbella karoonensis Q41(T) (98.89 %), Kribbella aluminosa HKI 0478(T) (98.86%) and Kribbella hippodromi S1.4(T) (98.85%), similarities to other type strains of species of the genus Kribbella were found to be less than 98.7 %. Phylogenetic analyses using the 16S rRNA gene sequence and multilocus sequence analysis using the concatenated gene sequences of the gyrB, rpoB, recA, relA and atpD genes all showed that the strain formed a separate branch in the genus Kribbella. The cell wall contained LL-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H-4). Major fatty acids were iso-C-16:0, iso-C-14:0 and anteiso-C-15:0, these chemotaxonomic data supported the affiliation of strain NEAU-THZ27(T) to the genus Kribbella. The DNA G+C content was 68.0 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA-DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-THZ27(T) represents a novel species of the genus Kribbella, for which the name Kribbella jiaozuonensis sp. nov. is proposed. The type strain is NEAU-THZ27(T) (=CGMCC 4.7504(T) =DSM 105535(T)).