Standardization of the preanalytical phase of DNA extraction from fixed tissue for next-generation sequencing analyses.

Olga Kofanova,Camille Bellora, Sonia Garcia Frasquilho,Laurent Antunes,Gael Hamot,Conny Mathay,Kathleen Mommaerts,Arnaud Muller, Brian DeWitt,Fay Betsou

New Biotechnology（2020）

引用 16|浏览14

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摘要

•DNA extracted from both FFPE and PFPE tissue is fit-for-purpose for NGS.•Different tissue fixative/DNA extraction method combinations influence DNA quality.•Different tissue fixative/DNA extraction method combinations influence NGS results.•Combinatorial QC including %dsDNA, amplifiable DNA size and WGA DNA yield matters.

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关键词

CEN TS,DIN,EQA,FFPE,GIV,IHC,NCI,NGS,PF,PFPE,QC,RT,SPREC,UDG,WGA,% variants PF

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