LC-MS/MS analysis of the central energy and carbon metabolites in biological samples following derivatization by dimethylaminophenacyl bromide.

•Non-discriminant derivatization of biological samples using DmPABr reagent.•Single RPLC-MS/MS analysis in positive ionisation mode provides full coverage.•Absolute quantification utilising internal standards labelled by stable isotopes.•Proof of concept for amines, carboxylic acids and thiol-containing metabolites.•Application to a variety of biological matrices including urine and cell extracts.