Salidroside Suppresses Il-1 Beta-Induced Apoptosis In Chondrocytes Via Phosphatidylinositol 3-Kinases (Pi3k)/Akt Signaling Inhibition

Background: Salidroside, a natural dietary isothiocyanate, has been widely studied for its multiple effects, including promot- ing proliferation, anti-inflammation, and anti-apoptosis. In the present study, these effects of Salidroside were explored to assess whether it could prevent osteoarthritis (OA) in vitro.Material/Methods: The cytotoxic and proliferating effects of Salidroside on chondrocytes were detected by use of the Cell Counting Kit 8 assay. The expression levels of proteins were detected by Western blot. The cell apoptosis level was assessed by flow cytometry, and the levels of ROS, NO, caspase 3, and caspase 9 were assessed to evaluate the level of apoptosis. The expression level of pro-inflammatory factors was detected by ELISA.Results: Our results demonstrated that Salidroside promotes chondrocytes proliferation, inhibits IL-1 beta-induced apoptosis and inflammation, and scavenges reactive oxygen species (ROS) and NO of chondrocytes. Salidroside upregulates the level of Bcl-2 and downregulates the level of Bax. Salidroside also inhibits the production of caspase 3/9 and suppresses the phosphorylation of PI3K and AKT.Conclusions: Our results suggest that Salidroside prevents OA by its powerful pro-proliferating, anti-phlogistic, and anti- apoptotic effects by inhibiting PI3K/AKT.