Long non-coding RNA MNX1-AS1 promoted osteosarcoma proliferation and invasion via inhibiting KISS1.

OBJECTIVE: Recent studies revealed that long non-coding RNAs (IncRNAs) participate in the progression and development of many human diseases. In this work, we are committed to uncovering the association between IncRNA MNX1-AS1 and the development of osteosarcoma. PATIENTS AND METHODS: MNX1-AS1 expression of osteosarcoma cells and tissue samples was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Besides, we conducted functional assays including cell proliferation assay, colony formation and transwell assays. Furthermore, the negative correlation was found between the expression level of KISS1 and MNX1-AS1 in osteosarcoma tissues. RESULTS: MNX1-AS1 was higher-expressed in osteosarcoma samples than that in adjacent tissues. The abilities of proliferation and invasion were suppressed after MNX1-AS1 was knocked down in vitro. Moreover, KISS1 expression was upregulated at mRNA and protein level via silence of MNX1-AS1. Furthermore, the negative correlation was found between the expression level of KISS1 and MNX1-AS1 in osteosarcoma tissues. CONCLUSIONS: Our study demonstrated that MNX1-AS1 could enhance osteosarcoma cell proliferation and invasion by inhibiting KISS1, which might contribute to the therapy for osteosarcoma.