Multiplexed Detection Of Autoantibodies To Glycopeptides Using Microarray
IMMUNOPROTEOMICS: METHODS AND PROTOCOLS, 2ND EDITION(2019)
摘要
Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients' antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.
更多查看译文
关键词
Glycopeptide microarray, GalNAc, Sialylation, Serum biomarkers, Autoantibodies, Posttranslational modification, Glycosylation
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要