Metagenomics for broad and improved parasite detection: a proof-of-concept study using swine faecal samples

Claudia Wylezich, Ariane Belka,Dennis Hanke,Martin Beer,Sandra Blome,Dirk Höper

International Journal for Parasitology（2019）

引用 28|浏览6

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摘要

•Full-length 18S rRNA sequences were extracted from RNA-derived metagenomics datasets.•No primer bias hampers the analysis of data generated using unbiased metagenomics.•Ten different parasite species/subtypes (ST) were detected in 37 datasets.•Blastocystis ST 15 was discovered for the first known time in swine faeces.•Metagenomics has the potential to be used as a generic pathogen detection tool.

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关键词

Shotgun metagenomics,Parasite detection,Subtyping,Taxonomic assignment,False-positives,Intestinal protists,RIEMS,Pig faeces

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