Variations of Intracellular Ca 2+ Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells

IEEE Transactions on Biomedical Engineering(2019)

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摘要
Goal: Herein, the variations in transient Ca2+ mobilizations in HeLa cells exposed to a single, non-thermal pulsed electric field (PEF) are described. Methods: Three PEF waveforms categorized by pulse duration and intensity were used to deduce the kinetics involved in Ca2+ mobilization. A fast microscopic fluorescent imaging system and a fluorescent molecular probe were used to observe transient i...
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关键词
Electrodes,Electric fields,Fluorescence,Biomembranes,Ions,Calcium,Transient analysis
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