Sequence features of variable region determining physicochemical properties and polyreactivity of therapeutic antibodies.

•The process of development of therapeutic antibodies is complex.•Many antibodies fail to reach clinic because of inappropriate physicochemical behaviour.•Assessment of physicochemical parameters of antibodies and identification of problematic molecules is of great importance.•Physicochemical properties of antibodies relevant for development depend of the sequence features of variable region.•Discovered sequence correlates may contribute for early identification of antibodies with developability issues.