RADAR-seq: A RAre DAmage and Repair sequencing method for detecting DNA damage on a genome-wide scale.

•RADAR-seq identifies and detects genome-wide DNA damage events.•RADAR-seq mapped genome-wide ribonucleotides in wild-type and RNaseH2-deficient strains.•RADAR-seq mapped the genome-wide location of cyclobutane pyrimidine dimers in E. coli genomic DNA exposed to UV-radiation.•RADAR-seq tracked lagging strand synthesis and the origin of replication during E. coli DNA replication.