Hymenobacter Humicola Sp. Nov., Isolated From Soils In Antarctica

A set of three psychrotrophic bacterial strains was isolated from different soil samples collected at the deglaciated northern part of James Ross Island (Antarctica) in 2014. All isolates were rod-shaped, Gram-stain-negative, non-motile, catalase-positive and oxidase-negative, and produced moderately slimy red-pink pigmented colonies on Reasoner's 2A (R2A) agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, MALDI-TOF MS, chemotaxonomy methods and extensive biotyping using conventional tests and commercial identification kits was applied to the isolates in order to clarify their taxonomic position. Phylogenetic analysis based on the 16S rRNA gene showed that all isolates belonged to the genus Hymenobacter with the closest relative being Hymenobacter aerophilus DSM 13606(T), exhibiting 98.5% 16S rRNA gene pairwise similarity to the reference isolate P6312(T). Average nucleotide identity values calculated from the whole-genome sequencing data proved that P6312(T) represents a distinct Hymenobacter species. The major components of the cellular fatty acid composition were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-16:1 omega 5c, summed feature 4 (C-17:1 anteiso B/iso l), C-15:0 anteiso and C-15:0 iso. The menaquinone system of strain P6312(T) contained MK-7 as the major respiratory quinone. The predominant polar lipids were phosphatidylethanolamine and an unidentified phospholipid. Moderate to minor amounts of three unidentified polar lipids, four unidentified aminophospholipids, one unidentified glycolipid and one unidentified phospholipid were also present. Based on the obtained results, we propose a novel species for which the name Hymenobacter humicola sp. nov. is suggested, with the type strain P6312(T) (=CCM 8763(T)=LMG 30612(T)).