Improving homology-directed repair efficiency in human stem cells.

William C Skarnes,Enrica Pellegrino,Justin A McDonough

Methods（2019）

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摘要

•Human induced pluripotent stem cells provide an ideal model cell.•CRISPR technology for ‘scarless’ editing of single nucleotide variants.•Improved protocol enhances efficiency of homology-directed repair.•Commercially sourced reagents ensure reproducible results.

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