Efficient production of gluten hydrolase Kuma030 in E. coli by hot acid treatment without chromatography

Houquan Liu,Xian Fan,Haoyue Song,Xiaoyun Hu,Guimin Zhang,Chan Yu,Li Yi

Enzyme and Microbial Technology（2019）

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摘要

•SUMO as fusion protein prompted the folding and overexpression of Kuma030 in E. coli.•A non-affinity chromatography strategy was developed for fast purification of active Kuma030.•The purified Kuma030 was functional to efficiently degrade gluten in food samples.

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关键词

Kumamolisin,Fusion expression,Non-affinity chromatography purification,Gluten degradation

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