ROC and Anova Analysis on Human Macrophage THP 1 Gene

Objective: This study aimed to investigate the activation of human macrophages THP-I and its intracellular activation mechanism induced by AHP-II (Acanthopanax giraldii Harms polysaccharide). Methods: ELISA and real-time PCR was performed to detect the expression of cytokines (IL-6, TNF-alpha, IL-10, IL-1 beta) in THP-I stimulated by AHP-II, then determine the concentration of NO and ROS, detect the killing activity to HepG2 of THP-I with CCK-8, and measure the expression of MHC-II/CD80/CD86 receptors on THP-I cell surface with Flow Cytometry, and then Western blotting was performed to detect MAPK protein expression absense with ST2825 (MYD88 inhibitor) or not. Results: AHP-II significantly increased the mRNA and protein levels of IL-6, TNF-alpha, IL-10, IL-1 beta, promoted the release of NO and ROS, increased the killing activity of THP-I to HepG2, promotes the expression of MHC-II, CD80, CD86 receptors on the surface of THP-I, also activates the JNK signaling pathway by phosphorylating JNK protein, and the secretion of IL-1 beta is decreased when ST2825 treated, meanwhile the phosphorylation level of JNK protein is decreased. Conclusion: AHP-II can activate human macrophage THP-I through MYD88/JNK signaling pathway.