Novel xylanolytic triple domain enzyme targeted at feruloylated arabinoxylan degradation

Jesper Holck,Demi T. Djajadi,Jesper Brask,Bo Pilgaard,Kristian B.R.M. Krogh,Anne S. Meyer,Lene Lange,Casper Wilkens

Enzyme and Microbial Technology（2019）

引用 16|浏览17

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摘要

•The three domains of CE1-GH62-GH10 act in synergy to degrade wheat arabinoxylan.•CE1-GH62-GH10 and a mix of the catalytic domains display comparable activity levels.•The three domains all unfolds at 71.0 ± 0.05°C when linked together.•CE1 and GH62 displayed lower thermostability than when linked together with GH10.

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关键词

4NPA,4NPX,ABF,Araf,AX,β-d-1,(CE),DNS,EPS,FA,FAE,GH,Tm,XOS,WAX-I

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