Recording temporal data onto DNA with minutes resolution
bioRxiv(2019)
摘要
Recording biological signals can be difficult in three-dimensional matrices, such as tissue. We present a DNA polymerase-based strategy that records temporal biosignals locally into DNA to be read out later, which could obviate the need to extract information from tissue on the fly. We use a template-independent DNA polymerase, terminal deoxynucleotidyl transferase (TdT) that probabilistically adds dNTPs to single-stranded DNA (ssDNA) substrates without a template. We show that , the dNTP-incorporation preference of TdT changes with the presence of Co, Ca, Zn and temperature. Extracting the signal profile over time is possible by examining the dNTP incorporation preference along the length of synthesized ssDNA strands like a molecular ticker tape. We call this TdT-based untemplated recording of temporal local environmental signals (TURTLES). We show that we can determine the time of Co addition to within two minutes over a 60-minute period. Further, we can estimate the rise and fall of an input Co pulse to within five minutes. simulations run based on our data predict TURTLES can record pulses as short as 1 minute and could record up to 10 pulses with high accuracy. TURTLES has 200-fold better temporal resolution than other DNA-based recording techniques.
更多查看译文
关键词
temporal data,dna,minutes
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络