NAFAMOSTAT MESYLATE, A BROAD SPECTRUM SERINE PROTEASE INHIBITOR, REDUCES INTRAPERITONEAL ADHESION FORMATION IN A MURINE CAECAL LIGATION AND PUNCTURE MODEL FOR SEPSIS

A) A schematic drawing depicting components that were manipulated in the gut inflammation-on-a-chip.AP, apical, BL, basolateral, LPS, lipopolysaccharide, PBMC, peripheral blood mononuclear cell.(B) Disruption of intestinal epithelial morphology in response to DSS treatment.(C) Oxidative stress of the intestinal epithelium in different intestinal microenvironments, visualized by a fluorogenic probe CellROX.(D) Recruitment of immune cells in healthy and barrier dysfunctional epithelium.Physiological concentration of LPS (10 ng/ mL) was used.Green indicates PBMC and gray indicates intestinal epithelium.(E) Treatment of probiotics, VSL#3, before the barrier disruption maintains tight junction (ZO-1) and mucus production (WGA), but post-treatment of the probiotics did not ameliorate pathological situations and caused bacterial translocation.(F) Secretion of pro-inflammatory cytokines in pre-and post-probiotic treatment.*P<0.05,bars, 50 μm. Su1019