Genetic dissection of grain elements predicted by hyperspectral imaging associated with yield-related traits in a wild barley NAM population

•Hyperspectral imaging enables reliable estimation of grain nutrient concentrations.•Wild barley offers a broad variation for grain nutrient concentrations.•Identification of genomic regions associated with nutrient concentrations.•QTL Hotspots simultaneously affect multiple grain nutrients.•Wild HvGA20ox2 alleles increase several grain nutrients without yield loss.