Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases
Organic & Biomolecular Chemistry(2019)
摘要
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial beta 1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-beta-LacNAc from lactose using beta 1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.
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关键词
enzymatic synthesis,lactose
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