miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways.
MOLECULAR MEDICINE REPORTS(2019)
摘要
The aim of the present study was to explore the underlying mechanism of microRNA-338-3p (miR-338-3p) in lung cancer cell (A549) invasion and proliferation. A microarray assay showed that miR-338-3p upregulated 216 and downregulated 147 genes in A549 cells, and the differentially expressed genes were enriched for several signaling pathways, including AKT and beta-catenin signaling pathways. Western blotting results showed that phosphorylated (p)-AKT at Ser473 and at Thr308 and p-beta-catenin at Ser552 were downregulated. Inhibiting AKT signaling pathway decreased beta-catenin signaling pathway. Overexpression of beta-catenin promoted the invasion of A549 cells. In addition, miR-338-3p showed inhibitory effect on the primary tumor developed from Kras(G12D) mice. Together, the data of the present study support that miR-338-3p inhibits lung cancer cell invasion and proliferation by downregulating AKT/beta-catenin signaling pathway. The present findings supported the potential use of miR-338-3p in the treatment of lung cancer.
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关键词
A549,AKT,invasion,microRNA-338-3p
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