LH SIGNAL IN TESTIS CONTRIBUTES TO THE MAINTENANCE OF INTERSTITIAL FLUID HOMEOSTASIS

You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology (MP75)1 Apr 2019MP75-04 LH SIGNAL IN TESTIS CONTRIBUTES TO THE MAINTENANCE OF INTERSTITIAL FLUID HOMEOSTASIS Satoshi Nozaki*, Shoiciro Iwatsuki, Tomoki Takeda, Hiroki Kubota, Hiroyuki Kamiya, Shoichi Sasaki, Yukihiro Umemoto, and Takahiro Yasui Satoshi Nozaki*Satoshi Nozaki* More articles by this author , Shoiciro IwatsukiShoiciro Iwatsuki More articles by this author , Tomoki TakedaTomoki Takeda More articles by this author , Hiroki KubotaHiroki Kubota More articles by this author , Hiroyuki KamiyaHiroyuki Kamiya More articles by this author , Shoichi SasakiShoichi Sasaki More articles by this author , Yukihiro UmemotoYukihiro Umemoto More articles by this author , and Takahiro YasuiTakahiro Yasui More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000557266.43719.78AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: During microsurgical testicular sperm extraction, many cases exhibit increased leakage of testicular interstitial fluid. The interstitial tissue mostly comprises Leydig cells that are regulated by the luteinizing hormone (LH). The study aimed to investigate the relationship between LH signals and interstitial edema using mice with impeded sperm production ability in which LH signaling of Leydig cells had been suppressed. METHODS: We used 6-week-old C57BL/6J mice to create a leuprorelin group (L group) and a control group (C group). L group was subcutaneously injected with the LHRH agonist leuprorelin acetate at 30 mg/kg and C group had received subcutaneous injections of a physiological saline solution. Testes were extracted at 4 and 8 weeks after administration and testicular weights were measured. Hematoxylin- and eosin-stained specimens were used to measure seminiferous tubule diameter and interstitial tissue area per testicular weight. We measured the proportion of seminiferous tubules in which the elongated spermatids had disappeared and evaluated sperm production dysfunction. RESULTS: Testicular weight was significantly lower (P<0.01) in L group (79.7 and 81.4 mg at 4 and 8 weeks after administration, respectively) than in C group (101.5 and 103.6 mg, respectively). Seminiferous tubule diameter was shorter in L group (174.2 and 166.7µm at 4 and 8 weeks, respectively) than in C group (182.5 and 176.3µm, respectively). Interstitial tissue area per testicular weight was significantly higher (P<0.01) in L group (1.79 and 1.13 at 4 and 8 weeks, respectively) than in C group (1.13 and 1.03, respectively). In addition, sperm production ability was impaired in L group but normal in C group. The proportion of seminiferous tubules in which elongated spermatids had disappeared in L group was 8.3 and 20.3% at 4 and 8 weeks, respectively, but remained at 0% for C group at both 4 and 8 weeks. CONCLUSIONS: This study first revealed that LH signals are involved in the homeostasis of testicular interstitial fluid and that abnormal LH signals can impair sperm production. Results suggest that regulating LH signals could improve testicular interstitial edema and sperm production ability. Source of Funding: none Nagoya, Japan© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e1083-e1083 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Satoshi Nozaki* More articles by this author Shoiciro Iwatsuki More articles by this author Tomoki Takeda More articles by this author Hiroki Kubota More articles by this author Hiroyuki Kamiya More articles by this author Shoichi Sasaki More articles by this author Yukihiro Umemoto More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement PDF downloadLoading ...