Abstract 463: Elevated Oxidative Stress and Reduction of Hydrogen Sulfide (H2S) and Nitric Oxide (NO) in HIV Patients: Possible Role in Cardiovascular (CV) Disease

Background: HIV attacks the immune system and weakens the ability to fight infections/disease. HIV infection confers approximately two-fold higher risk of cardiac events compared with the general population. ADMA, high-sensitivity CRP (hs-CRP) and IL-6 are well-characterized biomarkers of cardiac events. H2S and NO exert potent antioxidant properties and are cardioprotective. The present study was undertaken to determine the levels of H2S, NO, and CV disease biomarkers in HIV patients. Methods: Plasma from both HIV patients and normal subjects were used for all assays. Oxidative stress was determined by analyzing the levels of advanced oxidation protein products and H2O2. Antioxidant levels were determined by measuring the levels of trolox equivalent capacity. ADMA, hs-CRP, and IL-6 were determined by using ELISA. The levels of H2S/sulfane sulfur and nitrite were determined in plasma by using gas chromatography and HPLC, respectively. Results: We observed a marked induction in the levels of oxidative stre...