New recombinant of Tomato leaf curl New Delhi virus infecting melon in Indonesia

The complete nucleotide sequence of Tomato leaf curl New Delhi virus detected from melon in Indonesia-[Indonesia:Yogya:Melon:2015] (ToLCNDV-[ID:YG:Mel:15]) was determined. ToLCNDV-[ID:YG:Mel:15] has bipartite DNAs, and both DNAs showed highest identity to ToLCNDV-[Indonesia:Java:Cucumber:2008] (ToLCNDV-[ID:JV:Cuc:08]). However in a phylogenetic analysis based on AV1, ToLCNDV-[ID:YG:Mel:15] clustered with Squash leaf curl China virus (SLCCNV) isolates. Recombination analyses revealed that ToLCNDV-[ID:YG:Mel:15] is a recombinant between ToLCNDV and SLCCNV. Thus, the virus is a new recombinant of ToLCNDV. The infectivity of the recombinant ToLCNDV-[ID:YG:Mel:15] isolate was confirmed, and the inoculation induced mosaic on melon, but not on tomato, the primary natural host of ToLCNDV.