A novel enzymatic biosensor for detection of intracellular hydrogen peroxide based on 1-aminopyrene and reduced graphene oxides

n electrochemical sensor for hydrogen peroxide (H_2O_2) detection was established using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine with rGO via - conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the - conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 M to 28.5 M and a low detection limit of 0.5 M with good stability and high selectivity, confirming that the sensor is well-suited for the detection of H_2O_2 during biological processes. Graphical Abstract Using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO), an enzymatic hydrogen peroxide ( H_2O_2) biosensor was established with wide linear range, good stability and high selectivity.