Abstract 515: Bariatric Surgery Reverses Vascular Pathology in Mice With Morbid Obesity and Type 2 Diabetes

Objective: The mechanism of improved insulin sensitivity and vascular function following bariatric surgery remains unclear. We assessed the role of bariatric surgery in coronary arteriolar function in murine type 2 diabetic mice. Approach and Results: The animals were randomly allocated into normal control (Lean) sham, Lean surgery, db/db sham, and db/db surgery (at 5, 10, 20 and 30 days post-operatively). Impaired vasodilation to acetylcholine (ACh) in db/db mice was partially restored by bariatric surgery. Ghrelin administration restored endothelium-dependent dilation in db/db mice, but surgery-induced restoration was disappeared by ghrelin antagonist. Additionally, the incubation of coronary arterioles with IL-6 prevented the restoration of ACh-induced vasodilation. Bariatric surgery attenuated plasma concentration and protein expression of IL-6 in db/db. Bariatric surgery increased protein expression of eNOS and SOD2 in db/db mice. Conclusions: Decreased IL-6 expression after bariatric surgery induce up-regulation of eNOS and SOD2, and down-regulation of iNOS and O 2 .− , leading to restoration of endothelial function in coronary arterioles in type 2 diabetes.