Real-time monitoring and control of CHO cell apoptosis by in situ multifrequency scanning dielectric spectroscopy

Fuduo Ma,An Zhang,David Chang,Orlin D. Velev,Kelly Wiltberger,Rashmi Kshirsagar

Process Biochemistry（2019）

引用 15|浏览6

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摘要

•Detected apoptosis in CHO cells with in situ scanning dielectric spectroscopy.•Demonstrated potential to recover from early apoptosis in a real CHO cell process.•Applied scanning dielectric spectroscopy to detect deviation in manufacturing batch.

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关键词

Dielectric spectroscopy,Apoptosis,Multi-frequency permittivity,Chinese Hamster Ovary (CHO) cell culture,Fed-batch,Feedback control

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