Free radical scavenging potential and antihaemolytic activity of methanolic extract of Pouteria campechiana (Kunth) Baehni. and Tricosanthes tricuspidata Linn

Antioxidants from natural sources exhibits high standards concerning their obligatory roles in the improvement of nutraceuticals, pharmaceuticals and cosmetic products. The present study was carried out to evaluate the in vitro antioxidant, anti haemolytic activity and FT – IR analysis of two medicinal plants namely, Pouteria campechiana and Tricosanthes tricuspidata. In vitro antioxidant activity of PCBE and TTME extract was screened by various antioxidant assays such as 1,1- diphenyl–2-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiozoline-6-sulfonic acid cation decolorization (ABTS)test, hydroxyl radical scavenging, inhibition of lipid peroxidation, superoxide scavenging assay, reducing power capacity and antihaemolytic activity assay. PCBE exhibited high anti radical activity in DPPH, ABTS, hydroxyl radical scavenging, lipid peroxidation and superoxide radical scavenging activity with the IC50 value of 41.1, 9, 220, 82 and 48.2 μg/ml respectively where as the TTME showed moderate activity with the IC50 value of 63.3, 47, 130, 161, 84 μg/ml respectively. Linear regression analysis of phenolic and flavonoid content and antioxidant assays for PCBE and TTME displayed a positive correlation (R > 0.931; R2 > 0.614) which indicate that polyphenols are considered as main antioxidants. The FT-IR spectroscopy analysis confirms the presence of alcohols, phenols, alkanes, amides, amino acids, carboxylic acids, nitro compounds and amines in both the extract. The results suggested that the phytochemicals in both the plants have potent antioxidant activities in correlation with their polyphenolics content.