Design and Characterization of a Novel Artificial Peroxidase

In this study, we reported on a novel mimetic peroxidase, deuterohemin-Ala-His-Glu, (Dh-A-H-E). The kinetic parameters of Dh-A-H-E suggested that it was a mimetic peroxidase and followed the ping-pong mechanism. Compared to horseradish peroxidase, Dh-A-H-E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh-A-H-E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.