Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)

Summary Background Stem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing. Objective This study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-β1, TGF-β2, Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages. Methods This study used ELISA method to measure the growth factors in CM-hATMSCs. Results FGF was the highest modulator among FFP and non-FFP-treated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P  Conclusion In summary, the highest concentration level found in CM-hATMSCs is FGF both in FFP and non-FFP-treated cells. FGF as growth factor composition in CM-hATMSCs has potential as wound healing and regeneration of cell and it can be used in anti-aging products.